MiR-21 Is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells

Breast cancer (BCa) is one of the leading health problems among women. Although significant achievements have led to advanced therapeutic success with targeted therapy options, more efforts are required for different subtypes tumors and according genomic, transcriptomic, proteomic alterations. This study underlines role microRNA-21 (miR-21) in metastatic MDA-MB-231 breast cells. Following knockout miR-21 from cells, which highest expression levels compared MCF-7 SK-BR-3 BCa a decrease epithelial-mesenchymal transition (EMT) via downregulation mesenchymal markers was observed. Wnt-11 critical target miR-21, related signaling axis altered stable associated increase Furthermore, release extracellular vesicles (EVs) significantly reduced KO alongside reduction relative export EV cargo, control We conclude that factor involved BCa. Future strategies could focus on its treatment cancer.